Description |
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Background: ChIP-seq is the primary technique used to investigate
genome-wide protein-DNA interactions. As part of this procedure, immunoprecipitated
DNA must undergo "library preparation" to enable subsequent high-throughput
sequencing. To facilitate the analysis of biopsy samples and rare cell populations,
there has been a recent ... read moreproliferation of methods allowing sequencing library
preparation from low-input DNA amounts. However, little information exists on the
relative merits, performance, comparability and biases inherent to these procedures.
Notably, recently developed single-cell ChIP procedures employing microfluidics must
also employ library preparation reagents to allow downstream
sequencing.
Keywords: HTS, NGS, Low-input, Micro-ChIP, Chromatin
immunoprecipitation.
Springer Open.read less
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Citation |
- Sundaram, Arvind, Timothy Hughes, Shea Biondi, Nathalie
Bolduc, Sarah K. Bowman, Andrew Camilli, Yap C. Chew, Catherine Couture, Andrew
Farmer, John P. Jerome, David W. Lazinski, Andrew McUsic, Xu Peng, Kamran Shazand,
Feng Xu, Robert Lyle, and Gregor D. Gilfillan. "A comparative study of ChIP-seq
sequencing library preparation methods." BMC Genomics 17, no. 3 (12, 2016):
1-12.
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