IL-1β and IL-10 levels in Peri-implant Crevicular Fluid in Patients with Peri-implantitis
Abstract: Aim & Hypothesis: This study's specific aims were: 1) To compare the levels of IL-1β and IL-10 in peri-implant crevicular fluid (PICF) between patients with healthy implants (HI) and patients with implants with peri-implantitis (PI), and 2) to compare the levels of IL-1β and IL-10 in PICF and their correlation with changes of clinical parameters (probing depth [PD], bleeding on probing [... read moreBOP]) in patients with PI before and after non-surgical mechanical debridement with peri-implant pocket irrigation with chlorhexidine digluconate (NSMD). The hypothesis was that levels of IL-1ß would be higher in PI vs. HI, and levels of IL-10 would be lower in PI vs. HI. Materials & Methods: Patients with implants that were HI or PI were recruited for the study. All participants underwent a clinical and radiographic evaluation. PICF was collected for ELISA analysis of levels of IL-1β and IL-10. PI participants received NSMD, returned after 4 weeks for peri-implant pocket irrigation, and again returned 4 weeks later, where PICF was collected for ELISA analysis of levels of IL-1β and IL-10, and clinical parameters were measured. Results: Ten patients in the HI group and 12 patients in the PI group participated. IL-10 measurements in all participants were below detection level and were not included. Neither the amount nor the concentration of IL-1ß was significantly different between PI and HI at baseline (p=0.076 and p=0.058, respectively). IL-1ß concentration was significantly higher in PI at follow-up compared to baseline (p=0.032). At baseline, PI had significantly deeper PD more clinical attachment loss (CAL), more BOP and a higher plaque index compared to HI (p=0.005, p<0.001, p<0.001, and p=0.035, respectively). When comparing PI between baseline and follow-up, at follow-up, PD (p<0.001), CAL (p=0.001), and BOP (p=0.006) were significantly lower. Although clinical parameters were negatively correlated with IL-1ß concentration at baseline, only BOP was statistically significant (rs = -0.516, p = 0.018). This trend continued at Visit 3, only none of the correlations was statistically significant. Conclusions: Based on our findings, NSMD for PI significantly improved clinical parameters, including PD, CAL, and BoP. There were no significant differences between levels of IL-1β in PICF between patients with HI and patients with PI. Future studies including larger samples and a longer period of observation after peri-implant therapy may allow us to draw safer conclusions about the therapeutic value of NSMD and identify biomarker profiles that can aid in the diagnosis and treatment of PI.
Thesis (M.S.)--Tufts University, 2017.
Submitted to the Dept. of Periodontology.
Advisor: Evangelos Papathanasiou.
Committee: Bjorn Steffensen, Driss Zoukhri, and Matthew Finkelman.
Keywords: Dentistry, and Biology.read less