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Abstract: This PhD
dissertation focuses on the study of the effects of magnetic exposure on biological
systems using amperometry techniques and viability assays. In our prior work based on
the cyclotron resonance model, chromaffin cells in physiological saline and Ca2+-free
media were exposed for 5 minutes to a 2.7 μT magnetic field, with frequency sweeps
going from 30-60 Hz (targeting several ... read moreions involved in exocytosis) and 44-48 Hz
(targeting specifically Ca2+ ions), with noticeable effects on exocytosis. The present
study extended the work on chromaffin cells by covering frequency sweeps for different
ions, manipulating the time of exposure and the strength of the magnetic field.
Furthermore, amperometry was conducted on acute coronal brain slices, to demonstrate
that the recorded effects could be measured on neuronal tissue. The viability of
chromaffin cells and primary neuronal cultures exposed to magnetic fields was also
addressed. The results demonstrate that cellular exocytosis is sensitive to the
frequency of the magnetic field it is exposed to, the strength of the magnetic field and
the duration of exposure. No significant effects were established with regards to the
viability of the cells exposed to magnetic
fields.
Thesis (Ph.D.)--Tufts University,
2013.
Submitted to the Dept. of Electrical
Engineering.
Advisors: Joseph Noonan, and Emmanuel
Pothos.
Committee: George Sergiadis, and Brian
Tracey.
Keywords: Electrical engineering,
Neurosciences, and Electromagnetics.read less
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