Background: Studies on liver regeneration following partial hepatectomy
(PH) have identified several microRNAs (miRNAs) that show a regulated expression
pattern. These studies involve major surgery to access the liver, which is known to
have intrinsic effects on hepatic gene expression and may also affect miRNA screening
results. We perfo... read morermed two-third PH or sham laparotomy (SL) in Wistar rats to
investigate the effect of both procedures on miRNA expression in liver tissue and
corresponding plasma samples by microarray and qRT-PCR analyses. As control groups,
non-treated rats and rats undergoing anesthesia only were used.
Werner, Wiebke, Hannes Sallmon, Annekatrin Leder, Steffen
Lippert, Anja Reutzel-Selke, Mehmet Haluk Morgül, Sven Jonas, Christof Dame, Peter
Neuhaus, John Iacomini, Stefan G. Tullius, Igor M. Sauer, and Nathanael Raschzok.
"Independent effects of sham laparotomy and anesthesia on hepatic microRNA expression
in rats." BMC Research Notes 7, no. 1 (12, 2014): 1-8.