Re-establishing the Shear Bond Strength at the Dentin-Resin Interface After Blood Contamination
Abstract: Aim The aim of this in-vitro study was to compare the effectiveness of four decontamination methods in re-establishing the shear bond strength (SBS) to the control group SBS at the dentin-resin interface after fresh human blood contamination on etched dentin. Hypothesis Blot-drying alone would result in the most significant drop in SBS, whereas re-etching and the application of 2% chlorh... read moreexidine gluconate would have similar effectiveness in re-establishing SBSs at the dentin-resin interface, but more than only washing after blood contamination. Materials and Methods Occlusal surfaces of sixty mounted virgin molars and premolars were ground to expose dentin and divided into five groups (n=12) according to surface treatment. G1 (control) had no contamination. The other groups were contaminated with fresh human blood after acid-etching the dentin (ScotchbondTM etchant), and subjected to different post-contamination protocols; blot-drying only (G2); washing and drying alone (G3); re-etching (G4); and application of 2% chlorhexidine gluconate (Consepsis®) (G5). Scotchbond Universal Adhesive was applied followed by the direct placement of FiltekTM Supreme Ultra Flowable Restorative resin composite. The samples were stored in a distilled water at 370C for 24 hours. SBSs were measured and mode of failure was assessed microscopically (x10). For assessing the SBS, Dunn's post-hoc tests (with the Bonferroni correction) were conducted after the Kruskal-Wallis test, Fisher's exact test and post-hoc tests with the Bonferroni correction were used to establish significant differences between the modes of failures of the groups. Results The Kruskal-Wallis test indicated a significant difference between the groups (p < 0.001). Dunn's post-hoc tests (with the Bonferroni correction revealed that G1 produced significantly higher SBS than each of the other groups (p 0.002). No significant differences were found between the contaminated groups (p > 0.005), but a trend of G2
Thesis (M.S.)--Tufts University, 2017.
Submitted to the Dept. of Pediatric Dentistry.
Advisor: Gerard Kugel.
Committee: Mohamed Hassan, Matthew Finkelman, Cheen Loo, Driss Zoukhri, and Jeffrey Daddona.
Keyword: Dentistry.read less