Effect of Ethylenediaminetetraacetic Acid (EDTA) on the Bactericidal Properties of Sodium Hypochlorite Irrigation Solution. An in vitro Study.
Abstract: Aim: Sodium hypochlorite (NaOCl) is a potent antimicrobial agent that has the ability to eliminate microbes from root canals. Ethylenediaminetetraacetic Acid (EDTA) has been recommended as an adjunctive irrigant in root canal therapy to remove inorganic material from dentinal walls. However, the addition of EDTA to NaOCl causes a potential interaction between the two chemicals resulting ... read morein reduced pH, an increase in hypochlorous acid and chlorine gas and subsequent loss of the active hypochlorite ions. The purpose of this study was to determine if addition of EDTA interferes with the bactericidal properties of NaOCl against certain microorganisms found in root canal. Hypothesis: EDTA, added to NaOCl in a 1:1 ratio, will inhibit the bactericidal properties of NaOCl against root canal microorganisms. Material and Method: Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis (P. gingivalis) were chosen for this study and cultured per ATCC recommendations. Bacterial suspensions were incubated for 30s, 3 min or 30 min in Brain Heart Infusion broth (Difco) (negative control group), with 5.25% or 2.625% NaOCl (positive control groups), 17% EDTA, or 5.25% NaOCl mixed with 17% EDTA (1:1 ratio; test group). Total viable counts were determined using the serial dilution and plate counting method. Descriptive statistics (means and standard deviations) were computed. Survival percentages were analyzed via 95% confidence intervals. Results: The application of NaOCl (5.25%) or NaOCl (2.625%) diluted with H2O completely eliminated of both E. faecalis and P. gingivalis, at all-time points tested. EDTA alone did not affect the survival of either bacterium at any time point tested. Importantly, addition of EDTA to NaOCl significantly decreased the bactericidal properties of NaOCl against both bacteria at all time point even after a 30 minute exposure. Conclusion: Under the limitations of the present study, we concluded that EDTA, used at a 1:1 ratio, interferes with the bactericidal properties of NaOCl.
Thesis (M.S.)--Tufts University, 2015.
Submitted to the Dept. of Endodontics.
Advisor: Driss Zoukhri.
Committee: Robert Amato, Jennifer Bassett-Midle, Daniel Green, and Michael Malamy.
Keyword: Dentistry.read less