Development of a DNA Assay Based on Digital Quantification of Single Molecules by Total Internal Reflection Fluorescence MicroscopyLacy, Michael M.
Single molecule detection schemes promise the ability to reach the ultimate limit of detection: one molecule. In this project we use Total Internal Reflection Fluorescence Microscopy (TIRFM) to detect individual biomolecules for quantification. TIRFM has the advantage of delivering high contrast images and has become a standard method for the optical detection of single molecules. Her... read more
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