Development of novel methods to characterize restriction endonuclease kinetics and specificity

Wilburn, Frances C.

Restriction endonucleases are enzymes that cleave double stranded DNA and are widely used in recombinant DNA technology, yet much about their mechanisms of action is poorly understood. It is the goal of this research to fill the information gap on restriction endonucleases and increase our understanding of how these enzymes work by addressing the lack of kinetic data on type II restr... read more

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