Identifying the Red Blood Cell interacting molecule of Babesia microti.
Empitu, Maulana.
2015
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Abstract: Babesia
microti is an emerging human pathogen that infects red blood cells (RBCs) and causing
severe disease in immunocompromised population. Despite its clinical importance,
relatively little is known about the molecular mechanisms of RBC invasion by B. microti.
We employed phage display technology to identify molecules that mediate parasite-host
interaction of B. microti. From this ... read morescreen, we identified B. microti secreted antigen 1
(BmSA1) as a molecule that binds to human RBCs. Recombinant BmSA1 protein expressed in
E. coli bound to human and mouse RBCs in vitro. The RBC binding of BmSA1 was not
sensitive to neuraminidase, trypsin or chymotrypsin treatment of human RBCs. The binding
of BmSA1 was slightly less to the mouse reticulocytes enriched RBCs as compared to
normal RBCs, consistent with the previous observation that B. microti primarily invades
mature RBCs in mice. Taken together, we propose that BmSA1 is a parasite ligand utilized
by B. microti to bind host RBCs upon invasion
process.
Thesis (M.S.)--Tufts University, 2015.
Submitted to the Dept. of Pharmacology & Experimental Therapeutics.
Advisor: Athar Chishti.
Committee: Emmanuel Pothos, and John Castellot.
Keywords: Pharmacology, Parasitology, and Biochemistry.read less - ID:
- kp78gt59v
- Component ID:
- tufts:20319
- To Cite:
- TARC Citation Guide EndNote
