Observing single enzyme molecules interconvert between activity states upon heating.

Rojek, Marcin J.
Walt, David R.
2014

In this paper, we demonstrate that single enzyme molecules of β-galactosidase interconvert between different activity states upon exposure to short pulses of heat. We show that these changes in activity are the result of different enzyme conformations. Hundreds of single β-galactosidase molecules are trapped in femtoliter reaction chambers and the individual enzymes are subjected to short heating ... read more

Subjects
Enzymes.
Enzyme kinetics.
Tufts University. Department of Chemistry.
Permanent URL
http://hdl.handle.net/10427/010295
Original publication
Rojek MJ, Walt DR (2014) Observing Single Enzyme Molecules Interconvert between Activity States upon Heating. PLoS ONE 9(1): e86224. doi:10.1371/journal.pone.0086224.
ID: tufts:18960
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