Observing single enzyme molecules interconvert between activity states upon heating.Rojek, Marcin J.
Walt, David R.
In this paper, we demonstrate that single enzyme molecules of β-galactosidase interconvert between different activity states upon exposure to short pulses of heat. We show that these changes in activity are the result of different enzyme conformations. Hundreds of single β-galactosidase molecules are trapped in femtoliter reaction chambers and the individual enzymes are subjected to short heating ... read more
- Enzyme kinetics.
- Tufts University. Department of Chemistry.
- Permanent URL
- Original publication
- Rojek MJ, Walt DR (2014) Observing Single Enzyme Molecules Interconvert between Activity States upon Heating. PLoS ONE 9(1): e86224. doi:10.1371/journal.pone.0086224.